Journal: Experimental mechanics

Article Title: In situ measurement of native extracellular matrix strain

doi: 10.1007/s11340-019-00499-y

Figure Lengend Snippet: Experimental procedure. a. Decellularization of murine embryonic forelimbs allows the visualization of the extracellular matrix in its native conformation using confocal microscopy, after staining for ECM of interest. Decellularized forelimbs are imaged with an inverted confocal microscope while submerged in PBS. Embryo decellularization panels adapted from [12]. b. Volume images obtained before (cyan) and after load (magenta), corresponding to the same area of the digit, are correlated to extract displacements with fast iterative digital volume correlation (FIDVC). Displacements are analyzed and plotted in color as vector fields. Strain is calculated by fitting each set of displacement values to polynomial functions and then calculating their derivatives analytically, indicated by u(x) and u’(x).

Article Snippet: The intensity arrays of the reference and compressed configurations were compared using the MATLAB-based FIDVC algorithm developed by Bar-Kochba et al. , to extract the displacement fields of the ECM networks [ 13 ].

Techniques: Confocal Microscopy, Staining, Microscopy, Plasmid Preparation

Journal: Experimental mechanics

Article Title: In situ measurement of native extracellular matrix strain

doi: 10.1007/s11340-019-00499-y

Figure Lengend Snippet: Error quantification and analysis. a. Representative displacement vector field resulting from the fast-iterative digital volume correlation (FIDVC) of the same configuration imaged twice, without the application of load for WGA (top) and FBN2 (bottom). Displacement magnitude (in the order of picometers) is shown with color in the vector field. b. Representative absolute displacement error distribution for fibrin gel compression experiment and FIDVC. c. Displacement fields of the fibrils (top) and the fluorescent particles (bottom) in the fibrin gels generated for FIDVC displacement validation. d. Representative absolute difference between the FIDVC-calculated displacement of fluorescent particles and the adjacent fibrils.

Article Snippet: The intensity arrays of the reference and compressed configurations were compared using the MATLAB-based FIDVC algorithm developed by Bar-Kochba et al. , to extract the displacement fields of the ECM networks [ 13 ].

Techniques: Plasmid Preparation, Generated

Journal: Experimental mechanics

Article Title: In situ measurement of native extracellular matrix strain

doi: 10.1007/s11340-019-00499-y

Figure Lengend Snippet: Gaussian filtered FIDVC displacement components at 3 representative positions (indicated by different colors) in sample B, including their corresponding polynomial fit. Negative z-displacements are in the direction of the applied load.

Article Snippet: The intensity arrays of the reference and compressed configurations were compared using the MATLAB-based FIDVC algorithm developed by Bar-Kochba et al. , to extract the displacement fields of the ECM networks [ 13 ].

Techniques:

Journal: Experimental mechanics

Article Title: In situ measurement of native extracellular matrix strain

doi: 10.1007/s11340-019-00499-y

Figure Lengend Snippet: FIDVC absolute displacement error

Article Snippet: The intensity arrays of the reference and compressed configurations were compared using the MATLAB-based FIDVC algorithm developed by Bar-Kochba et al. , to extract the displacement fields of the ECM networks [ 13 ].

Techniques: